Comparable effects of DIGIBIND and DigiFab in thirteen digoxin immunoassays.
نویسندگان
چکیده
tients. Clin Chem 1995;41:256–63. 9. Niederwieser G, Bonelli RM, Brunner D, Neubauer M, Buchinger W, Reisecker F, et al. Diagnostic accuracy of an agarose gel electrophoretic method in multiple sclerosis. Clin Chem 2001;47:144. 10. Cavuoti D, Baskin L, Jialal I. Detection of oligoclonal bands in cerebrospinal fluid by immunofixation electrophoresis. Am J Clin Pathol 1998;109:585–8. 11. Richard S, Miossec V, Moreau JF, Taupin JL. Detection of oligoclonal immunoglobulins in cerebrospinal fluid by an immunofixation-peroxidase method. Clin Chem 2002;48:167–73. 12. Sellebjerg F, Christiansen M. Qualitative assessment of intrathecal IgG synthesis by isoelectric focusing and immunodetection: interlaboratory reproducibility and interobserver agreement. Scand J Clin Lab Invest 1996;56:135–43.
منابع مشابه
Serum digoxin in the presence of digibind: determination of digoxin by the Abbott AxSYM and Baxter Stratus II immunoassays by direct analysis without pretreatment of serum samples.
We have reevaluated the feasibility of using direct immunochemical methods to track free digoxin in patients receiving Digibind. We report here that results obtained by the Stratus II and AxSYM immunoassays on patients receiving digoxin (without Digibind), digoxin-fortified serum samples supplemented with Digibind, and a digitoxic patient treated with Digibind, show no clinically significant bi...
متن کاملEffect of Indian Ayurvedic medicine Ashwagandha on measurement of serum digoxin and 11 commonly monitored drugs using immunoassays: study of protein binding and interaction with Digibind.
CONTEXT Ashwagandha, a popular Ayurvedic medicine, is now available in the United States. Alkaloids found in this herb have structural similarity with digoxin. OBJECTIVE To study potential interference of Ashwagandha with serum digoxin measurement by immunoassays. Potential interference was also investigated with immunoassays for 11 other commonly monitored drugs. In addition, interaction of ...
متن کاملMonitoring of unbound digoxin in patients treated with anti-digoxin antigen-binding fragments: a model for the future?
Digoxin is one of the few therapeutic drugs for which antidotal therapy is available (1). Administration of Fab fragments has successfully been used to reverse the effects of life-threatening digoxin overdoses for over 20 years. Digoxin-specific Fab fragments are produced by cleaving sheep digoxin-specific IgG with papain to form two Fab fragments (50 000 Da each) and one 50 000-Da Fc fragment ...
متن کاملNeutralization of free digoxin-like immunoreactive components of oriental medicines Dan Shen and Lu-Shen-Wan by the Fab fragment of antidigoxin antibody (Digibind).
Dan Shen and Lu-Shen-Wan, traditional Chinese medicines used as remedies for heart diseases, demonstrate digoxin-like immunoreactivity. The digoxin-like immunoreactive components of Lu-Shen-Wan show approximately 55% protein binding, while Dan Shen demonstrates concentration-dependent protein binding (68% bound at lower concentrations but only 25% bound at higher concentrations). Because Dan Sh...
متن کاملRapid detection of the active cardiac glycoside convallatoxin of lily of the valley using LOCI digoxin assay.
OBJECTIVES To explore the luminescent oxygen channeling technology-based digoxin immunoassay (LOCI digoxin assay) for rapid detection of lily of the valley extract and convallatoxin. The potential in vitro binding of convallatoxin with Digibind was also evaluated. METHODS Aliquots of a drug-free serum pool and a digoxin serum pool were supplemented with lily of the valley extract or convallat...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Clinical chemistry
دوره 48 9 شماره
صفحات -
تاریخ انتشار 2002